Volume 5, Issue 2, June 2019, Page: 51-55
Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury
Li Luo, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Xing Tu, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Xuexia Zhang, Department of Anesthesiology, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, People's Republic of China
Jing Liu, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Yilin Liu, School of Clinical Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Wenyan Zhao, School of Clinical Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Ke Niu, School of Clinical Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Junhua Yang, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Guoying Li, School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Mengxia Wang, Intensive Care Unit, Guangdong No. 2 Provincial People's Hospital, Guangzhou, People's Republic of China
Received: Mar. 20, 2019;       Accepted: Apr. 22, 2019;       Published: May 10, 2019
DOI: 10.11648/j.ajp.20190502.13      View  86      Downloads  17
Abstract
Objective: To investigate the neuroprotective effects of grape seed proanthocyanidin extract(GSPE)on a model of neonatal hypoxic ischemic encephalopathy (HIE), we investigated the changes in neuronal cells and astrocytes after pre-treatment with GSPE. Methods: Seven-day-old pups were randomly divided into sham, HI, and GSPE+HI groups. The HIE model was established using a modified Rice-Vannucci method. GSPE was injected intraperitoneally 20 min before surgery. The change in markers of neuronal cells and astrocytes (NeuN/GFAP) were detected by immunofluorescence and Western blot. Results: Compared with the sham group, the expression of NeuN in the HI group was significantly reduced, and the expression of NeuN was significantly increased after GSPE pre-treatment. The expression of GFAP was opposite to NeuN. Conclusion: Our study showed that GSPE pre-treatment significantly protected neurons and inhibited astrocyte over-proliferation. Therefore, we believe that GSPE is a potential drug for the treatment of HIE and can prevent brain damage caused by hypoxia and ischemia.
Keywords
Grape Seed Proanthocyanidin Extract, NeuN, GFAP, Neonatal Hypoxic-Ischemic Brain Injury
To cite this article
Li Luo, Xing Tu, Xuexia Zhang, Jing Liu, Yilin Liu, Wenyan Zhao, Ke Niu, Junhua Yang, Guoying Li, Mengxia Wang, Grape Seed Proanthocyanidin Extract Adjusts NeuN/GFAP in a Murine Model of Neonatal Hypoxic-ischemic Brain Injury, American Journal of Pediatrics. Vol. 5, No. 2, 2019, pp. 51-55. doi: 10.11648/j.ajp.20190502.13
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Liu G, Li Z G, Gao J S. Hypothermia in neonatal hypoxic-ischemic encephalopathy (HIE) [J]. Euro Review Medi Pharmacological Sci, 2017, 21: 50-53.
[2]
Rao R, Trivedi S, Distler A, et al. Neurodevelopmental Outcomes in Neonates with Mild Hypoxic Ischemic Encephalopathy Treated with Therapeutic Hypothermia [J]. Am J Perinatol, 2019.
[3]
Zhou X M, Liu J, Wang Y, et al. Silencing of long noncoding RNA MEG3 enhances cerebral protection of dexmedetomidine against hypoxic-ischemic brain damage in neonatal mice by binding to miR-129-5p [J]. J Cell Biochem, 2018.
[4]
Xu B, Xiao A J, Chen W, et al. Neuroprotective effects of a PSD-95 inhibitor in neonatal hypoxic-ischemic brain injury [J]. Molecular Neurobi, 2016, 53: 5962-5970.
[5]
Luo Z, Zhang M, Niu X, et al. Inhibition of the PI3K/Akt signaling pathway impedes the restoration of neurological function following hypoxic-ischemic brain damage in a neonatal rabbit model [J]. J Cell Biochem, 2019.
[6]
Devi A, Jolitha A B, Ishii N. Grape Seed Proanthocyanidin Extract (GSPE) and antioxidant defense in the brain of adult rats [J]. Medi Sci Mo, 2006, 12: BR124-BR129.
[7]
Liu B, Zhang H, Tan X, et al. GSPE reduces lead-induced oxidative stress by activating the Nrf2 pathway and suppressing miR153 and GSK-3β in rat kidney [J]. Oncotarget, 2017, 8: 42226-42237.
[8]
Rajput S, Sun L, Zhang N Y, et al. Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers [J]. Toxins, 2019, 11: 23.
[9]
Guo F, Hu Y, Niu Q, et al. Grape Seed Proanthocyanidin Extract Inhibits Human Esophageal Squamous Cancerous Cell Line ECA109 via the NF-κB Signaling Pathway [J]. Mediators Inflamm, 2018, 22: 6999-7012.
[10]
Hao J P, Shi H, Zhang J, et al. Role of GSPE in improving early cerebral vascular damage by inhibition of Profilin-1 expression in a ouabain-induced hypertension model [J]. Euro Review Medi Pharmacol Sci, 2018, 22: 6999-7012.
[11]
Sanna R S, Muthangi S, BK C S, et al. Grape seed proanthocyanidin extract and insulin prevents cognitive decline in type 1 diabetic rat by impacting Bcl-2 and Bax in the prefrontal cortex [J]. Metab Brain Dis, 2018: 1-15.
[12]
Fang M, Jiang H, Ye L, et al. Metformin treatment after the hypoxia-ischemia attenuates brain injury in newborn rats [J]. Oncotarget, 2017, 8: 75308-75325.
[13]
Al Mamun A, Yu H, Romana S, et al. Inflammatory Responses are Sex Specific in Chronic Hypoxic–Ischemic Encephalopathy [J]. Cell Trans, 2018.
[14]
Ye L, Feng Z, Doycheva D, et al. CpG-ODN exerts a neuroprotective effect via the TLR9/pAMPK signaling pathway by activation of autophagy in a neonatal HIE rat model [J]. Experi Neuro, 2018, 301: 70-80.
[15]
Jiang W, Guo M, Gong M, et al. Vitamin A bio-modulates apoptosis via the mitochondrial pathway after hypoxic-ischemic brain damage [J]. Mole Brain, 2018, 11: 14.
[16]
Birla H, Rai S N, Singh S S, et al. Tinospora cordifolia Suppresses Neuroinflammation in Parkinsonian Mouse Model [J]. NeuroMole Medi, 2019: 1-12.
[17]
Teismann P, Schulz J B. Cellular pathology of Parkinson’s disease: astrocytes, microglia and inflammation [J]. Cell Tissue Res, 2004, 318: 149-161.
Browse journals by subject